Screen Quest Fluo-8 去除培養(yǎng)基鈣檢測試劑盒

Screen Quest Fluo-8鈣檢測試劑盒是美國AAT Bioquest生產(chǎn)的鈣檢測試劑盒，鈣通量測定是用于篩選G蛋白偶聯(lián)受體（GPCR）的藥品發(fā)現(xiàn)中的優(yōu)選方法。 Screen Quest Fluo-8 NW鈣測定試劑盒提供均勻的熒光測定，用于檢測細(xì)胞內(nèi)鈣動員。表達(dá)感興趣的GPCR的細(xì)胞通過鈣預(yù)先加載我們專有的Fluo-8 NW，其可以穿過細(xì)胞膜。 Fluo-8 NW是可用于HTS篩選的亮鈣指示劑。一旦進(jìn)入細(xì)胞內(nèi)，F(xiàn)luo-8 NW的親脂性阻斷基團被非特異性細(xì)胞酯酶切割，產(chǎn)生帶負(fù)電荷的熒光染料，其停留在細(xì)胞內(nèi)，并且在與鈣結(jié)合時其熒光大大增強。當(dāng)用篩選化合物刺激細(xì)胞時，該受體表明細(xì)胞內(nèi)鈣的釋放，這極大地增加了Fluo-8 NW的熒光。其長波長，高靈敏度和100-250倍熒光增加的特性（當(dāng)它與鈣形成復(fù)合物時）使Fluo-8 NW成為測量細(xì)胞鈣的理想指標(biāo)。這個Screen Quest Fluo-8 NW鈣測定試劑盒提供了一種優(yōu)化的檢測方法，用于監(jiān)測G蛋白偶聯(lián)受體（GPCR）和鈣通道。該測定可以以方便的96孔或384孔微量滴定板形式進(jìn)行，并且易于適應(yīng)自動化。百螢生物是AAT Bioquest的中國代理商，為您提供的Screen Quest Fluo-8 去除培養(yǎng)基鈣檢測試劑盒*適合于難檢測細(xì)胞系*。

參考文獻(xiàn)

A Long-Acting PYY3--36 Analog Mediates Robust Anorectic Efficacy with Minimal Emesis in Nonhuman Primates
Authors: Shamina M Rangwala, Katharine D’Aquino, Yue-Mei Zhang, Lindsay Bader, Wilson Edwards, Songmao Zheng, Annette Eckardt, Ann Lacombe, Rebecca Pick, Veronica Moreno
Journal: Cell Metabolism (2019)

Oxidative Insults and Mitochondrial DNA Mutation Promote Enhanced Autophagy and Mitophagy Compromising Cell Viability in Pluripotent Cell Model of Mitochondrial Disease
Authors: Dar-Shong Lin, Yu-Wen Huang, Che-Sheng Ho, Pi-Lien Hung, Mei-Hsin Hsu, Tuan-Jen Wang, Tsu-Yen Wu, Tsung-Han Lee, Zo-Darr Huang, Po-Chun Chang
Journal: Cells (2019): 65

Prosocial effects of an oxytocin metabolite, but not synthetic oxytocin receptor agonists, in a mouse model of autism
Authors: Sheryl S Moy, Brian L Teng, Viktoriya D Nikolova, Natallia V Riddick, Catherine D Simpson, Amy Van Deusen, William P Janzen, Maria F Sassano, Cort A Pedersen, Michael B Jarstfer
Journal: Neuropharmacology (2019): 301--311